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Creative Biostructure is highly-experienced in custom mutagenesis libraries construction services for directed evolution. Site-saturation mutagenesis (SSM) can generate random or targeted mutations and screen the library of protein variants for functional alterations. Notably, our protein evolution and engineering platform holds many advantages for custom SSM libraries services, including extremely cost-efficient, fast delivery time, fewer variants to screen and, systematic identification of beneficial amino acid substitutions.
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Continuous evolution methods are developed recently to overcome some disadvantages of traditional protein evolution methods such as the requirements for discrete time- and labor- intensive steps during the construction and introduction of gene libraries, screening and selections, isolation of gene library members, etc. In continuous evolution methods, every steps of a protein evolution cycle can be performed continuously with no need for manual intervention. Most of the continuous evolution experiments have selected for replicative fitness of hosts under a continuous dilution, and have been ap
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plied for various evolution targets such as bacteria genomes for faster replication speed and resistance to antibiotics. The continuous evolution methods significantly improve the efficiency of protein evolution assays, along with the number of steps in the sequence space that can be explored in the screening for desired optimal protein variants.
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