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Structural biology incorporates techniques and principles of molecular biology, biochemistry and biophysics as a means of elucidating the molecular structure and dynamics of biologically relevant molecules. Recent progress in instrumentation has endorsed a new boost in structural biology as complex biological molecules can now be analyzed with unprecedented ease and efficiency. The three-dimensional structure of proteins and protein complexes provide great insights into the laws of life activities and mechanism of diseases, and thereby allowing rational design of novel diagnostic and therapeut
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ic agents. There are three main research techniques for structural biology: single crystal X-ray diffraction (SC-XRD), nuclear magnetic resonance (NMR) and cryo-electron microscopy (Cryo-EM). However, there is no “all-purpose” method since all three of them offer unique advantages as well as limitations.