Researchers usually prefer BLUeye or BLUelf Prestained Protein Ladder for Western Blotting because of its visual monitoring capabilities, precision, and convenience for protein analysis. Distinct color bands enhance the clarity of the steps in the gene expression workflow, from gel electrophoresis to membrane transfer. Western Blotting estimates the molecular weight of a protein by confirming its identity relative to protein ladders and assessing protein modifications and degradation.